FIG. 2.

MxA(L612K) is antivirally active. (A) Vero cells transfected with cDNAs encoding either wild-type MxA [MxA(wt)] or mutant protein MxA(L612K) or MxA(T103A) were infected with 50 PFU of THOV per cell. Cells were fixed 9 h later, and MxA proteins or viral proteins were detected by double immunofluorescence using a monoclonal antibody directed against MxA and a polyclonal antiserum directed against THOV antigens. (B) Quantitative analysis of infection experiments with THOV and VSV. The percentage of infected cells expressing either wild-type MxA [MxA(wt)], MxA(L612K), or MxA(T103A) is shown. Cells were infected with 10 PFU of VSV serotype Indiana per cell (15).