FIG. 4.
MxA(L612K) is rapidly degraded. COS-1 cells were transfected with expression plasmids coding for either MxA or MxA(L612K). Cells were then labeled for 2 h with 35 μCi of [35S]methionine per ml and chased for 2.5, 5, 7.5, and 10 h. (A) Wild-type and mutant MxA proteins were immunoprecipitated from cell lysates using a monoclonal antibody directed against MxA and analyzed by polyacrylamide gel electrophoresis and autoradiography. (B) PhosphoImager analysis of immunoprecipitated proteins. MxA(wt), wild-type MxA.