TABLE A1.

Inclusion and exclusion criteria for patients with amyotrophic lateral sclerosis and healthy control subjects.

Patients with ALS	Age‐ and sex‐matched healthy control subjects
Inclusion criteria
>18 years of age	>18 years of age
Neurologist‐confirmed diagnosis of a possible, probable or definite ALS according to rEE criteria
Ability to lie in an MRI scanner for 1 h	Able to lie in MRI scanner for 1 h
Exclusion criteria
Active other neurological or neuromuscular condition explaining the symptoms or a significant part of it	Neurological or neuromuscular conditions
Other neurological or neuromuscular conditions interfering with the examinations
Severe cervical spinal stenosis or other relevant disease of the spine or spinal cord	Severe cervical spinal stenosis or other relevant disease of the spine or spinal cord
Other severe chronic disease	Other severe chronic disease
Pregnancy	Pregnancy
General contraindications against MRI scanning (e.g. metal implants, pacemakers)	General contraindications against MRI
Not able to read the patient information due to language barriers	Not able to read the patient information due to language barriers
Major cognitive deficit impacting the ability to read and understand the patient information and/or to follow the instructions of the study personnel	Major cognitive deficit impacting the ability to read and understand the patient information and/or to follow the instructions of the study personnel

Abbreviation: ALS, amyotrophic lateral sclerosis; MRI, magnetic resonance imaging; rEE, revised El Escorial.