TABLE 1.
Virus | EOP for BAF30 (recA) with:
|
|||||
---|---|---|---|---|---|---|
pφXDJ | pα3DJ | pφXJ | pα3J | pφX/α3D | pα3/φXD | |
φX174 nullDb | 1.0 | <10−6 | 10−6 | 10−6 | 10−6 | 10−6 |
α32 nullDc | <10−6 | 1.0 | 10−6 | 10−6 | 10−6 | 10−6 |
Wild-type φX174 | 1.0 | 1.0–10−6 | 1.0 | 1.0 | 10−3–10−5 | 1.0–10−2 |
Wild-type α3 | 1.0–10−6 | 1.0 | 1.0 | 1.0 | 1.0–10−2 | 10−3–10−5 |
φX174 chiDr | 0.5 | 1.0–10−2 | 0.5 | 0.5 | 1.0 | 1.0–10−2 |
The construction of chimeric genes is depicted in Fig. 2.
Contains two amber mutations in the gene.
Contains a frameshift mutation in codon 20.