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. 2024 Jul 9;13(1):2373519. doi: 10.1080/2162402X.2024.2373519

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© 2024 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 2. — Tumor-derived fragments can be frozen and thawed showing comparable results with freshly excised fragments.

a) Scheme of the experiments including the freezing and thawing procedure. b and c) Comparative results following anti-PD-1 plus anti-CD137 stimulation of the fresh and frozen/thawed fragments. d) Flow cytometry results similar to Figure 1 comparing the frozen/thawed MC38-derived samples with those freshly excised. MDTFs: mouse-derived tumor fragments.