Figure 3.

IFNγ release into the supernatant does not predict the therapeutic response to anti-PD1 plus anti-CD137 mAb treatment in vivo.

a) Experiments in which mice bearing bilateral MC38-derived tumors were subjected to unilateral tumorectomy and subsequently treated with control mAb or the anti-PD-1 plus anti-CD137 combination. Excised and fragmented tumors were subjected to culture. b) Individual tumor follow-up classifying tumors as responding or not to treatment. c) IFNγ concentrations in the supernatants of the fragments stimulated ex vivo comparing responding and non-responding contralateral tumors in the mice. d) Serum concentrations of IFNγ at day 18, comparing in-vivo responding and non-responding tumors. e) Results from a multiplex array measuring 12 cytokines in the pooled supernatants of the fragments cultured in the presence of the indicated stimuli. Error bars in (e) indicate standard deviations. R: Responder. NR: non-responder.