FIG. 2.

Biological activities of K-Rev mutants. 293T cells were cotransfected with the pDM128/K-RRE indicator construct and expression plasmids encoding wild-type (WT) or mutant K-Rev. The parental pBC12/CMV expression plasmid served as a negative control (Neg). Induced CAT activities were assayed at ∼48 h after transfection as previously described (3). These data are normalized to the activity of wild-type K-Rev, which is arbitrarily set at 100. Results were also adjusted for nonspecific variability using the β-Gal internal control. Data shown represent the average of three independent experiments with standard deviation indicated.