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Immunofluorescence of cells incubated with the serum to the 75-kDa OG protein fraction. MA104 cells were fixed with paraformaldehyde and permeabilized with Triton X-100 (B and D) or not permeabilized (A and C). The cells were incubated with a 1:1,500 dilution of the preimmune (C and D) or hyperimmune (A and B) sera to the 75-kDa protein fraction for 90 min at 37°C and stained with a goat anti-rabbit immunoglobulin G coupled to fluorescein isothiocyanate.
