TABLE 1.
Effect of metabolic inhibitors, cell membrane cholesterol depletion, and OG on the infectivity of rotaviruses in MA104 cells
| Inhibitora | % Infectivity (SE)b of:
|
||||
|---|---|---|---|---|---|
| RRV | nar3 | Wa | Reovirus | Poliovirus | |
| None | 100 | 100 | 100 | 100 | 100 |
| PDMP (25 μg/ml) | 20 (2) | 40 (9.4) | 23 (3.8) | 95 (3) | 114 (0) |
| Tunicamycin (2 μg/ml) | 56 (2.5) | 48 (2.8) | —c | 91 (5.5) | 192 (15) |
| BenzylGalNAc (2 mM) | 101 (0.5) | 150 (4.8) | 147 (7.2) | 110 (4.5) | 108 (4.5) |
| OG (0.2%) | 41 (5.4) | 41 (2.4) | 39 (4.8) | 89 (2) | 199 (29) |
| β-Cyclodextrin (1 mM) | 9 (1.8) | 6 (2.3) | 5 (1.8) | 96 (0) | 95 (3) |
a
MA104 cell monolayers were incubated with the indicated concentration of inhibitor for 1 h (β-cyclodextrin), 24 h (tunicamycin), or 72 h (PDMP and benzylGalNAc) at 37°C or for 90 min (OG) at room temperature before virus infection.
b
SE, one standard error of the mean of at least three independent experiments carried out in duplicate.
c
The infectivity of Wa was inhibited by about 50% regardless of whether tunicamycin was added to the cells 24 h before or immediately after the virus adsorption; thus, this inhibition was considered nonspecific.