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. 2024 Jun 25;14(6):3171–3185. doi: 10.62347/MUKD2745

Figure 1.

Figure 1

Scheme of blood collection and NanoString analysis in tumor recurrence animal model. Twelve (four groups, n = 3/group) 6-week-old female BALB/c mice were included in this study. Six mice were subcutaneously transplanted with luciferase-tagged 4T1 (4T1-luc) mouse breast cancer cells (1×106 cells per mouse) into the 4th mammary fat pad. At week 3, one set of paired normal (n = 3) and tumor-bearing mice (n = 3) were euthanized, and PBMCs were collected. Another 3 tumor-bearing mice underwent tumor resection and suture surgery. The mice were monitored for tumor recurrence weekly using an IVIS imaging system. When a bioluminescent signal appeared (between weeks 5 and 7), tumor-recurrent paired mice were sacrificed for PBMC collection. PBMCs from 12 mice were subjected to NanoString analysis using nCounter® Mouse Myeloid Innate Immunity V2 panel (754 genes) and nCounter® Mouse PanCancer Immune Profiling Panels (770 genes).