TABLE 1.
Defining the A238L CaN binding motif by mutation analysisa
| Sequence | Amino acid residue
|
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 200 | 201 | 202 | 203 | 204 | 205 | 206 | 207 | 208 | 209 | 210 | 211 | 212 | 213 | |
| Wild type | W | F | K | K | K | P | K | I | I | I | T | G | C | K |
| Mutant | G | V | T | T | T | S | T | T | T | T | A | A | S | T |
| Non-CaN binding | 8 | 6 | 11 | 11 | 9 | 12 | 7 | 8 | 8 | 6 | 10 | 3 | 7 | 5 |
| CaN binding | 14 | 15 | 9 | 3 | 13 | 0 | 5 | 0 | 5 | 0 | 0 | 0 | 1 | 14 |
| Point −ve | S | T | T | A | G | |||||||||
| Point +ve | A | S | ||||||||||||
| Motifb | P | I | I | T | C/S | |||||||||
a
The number of times each residue was mutated during the random mutagenesis experiments in non-CaN-binding (n = 17) or CaN-binding (n = 19) mutants (residues not mutated in positive interactors are in boldface) and individual point mutations which prevented CaN binding (Point −ve) or bound CaN (Point +ve) are shown.
b
The deduced minimal CaN binding motif from A238L.