Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jul 10;15:5790. doi: 10.1038/s41467-024-49595-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Schematic of immune-oncology profiling workflow using clinical tumor samples. Created with BioRender.com. (APC: antigen-presenting cell). b Diagram of 18 genes included in tumor inflammation signature (TIS) in relation to immune activation (red text denotes genes that increase immune activity and blue denotes genes that decrease immune activation). Created with BioRender.com. c Images of digital quantification of CD3 IHC. Representative areas of whole slide images were selected for visualization, with the percentage of positive cells in the entire slide indicated. Scale bar (bottom left) is 100 μm. Images taken at 100x magnification. d Scatter plots of correlation between gene expression by NanoString and percentage of positive cells by IHC for CD3 and CD8 in matched tissue sections. Correlation and p-values were calculated using the Spearman correlation. (e) Violin plots of TIS scores across major pediatric tumor types from SickKids and PBTA datasets, compared to non-tumor brain controls. Boxes show the median and interquartile range (IQR) of the data with whiskers extending to ±1.5 IQR. p-values calculated using two-tailed T test with Holm adjustment. (HGG: high-grade glioma; LGG: low-grade glioma).