Fig 2.

Selective amplification of RNA from self-splicing transgenes by RT-LAMP. Time to threshold (Tt) values are shown for RT-LAMP reactions targeting both RNA and DNA or spliced, transgenic RNA. Organisms carrying transgenic expression vectors and precursor vectors lacking the transgene were tested:(A) E. coli carrying pKTM_IAC or the pUC19 precursor vector,(B) E. coli carrying pOrf142+gfp_mod or the pCN50wt precursor vector, and(C) S. aureus carrying pOrf142+gfp_mod or the pCN50wt precursor vector. Various cell equivalents of purified RNA and DNA templates were tested in triplicate, with results of four technical replicates from each test superimposed. Gray horizontal bars represent the mean for replicates.