Fig 1.

Apical-out porcine intestinal organoids are susceptible to TGEV. (A and B) Apical-out organoids were inoculated with TGEV, and organoids and supernatant were collected at the indicated times for viral load detection by RT-qPCR. (C and D) TGEV titers and N protein at the indicated times were detected by TCID₅₀ and Western blotting, respectively. (E) Apical-out organoids infected with TGEV for 48 h were stained with TGEV N monoclonal antibody; scale bar: 20 µm. All experiments were performed in triplicate.