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. 2024 May 16;15(6):e00679-24. doi: 10.1128/mbio.00679-24

Fig 5.

A set of scientific diagrams and assay results depict gene editing using CRISPR/Cas9 targeting phage genes, growth curves of bacterial cultures post-infection with phages at different MOIs, and bacterial lawns with and without gene deletions.

ΦNM1 gp11 is contributed to phage infection. (A) The scheme for sgRNA-guided, CRISPR/Cas 9-induced homologous recombination in the gp11 gene. (B) Growth curves of S. aureus RN4220 treated with ΦNM1 or ΦNM1Δgp11 at a multiplicity of infection (MOI) of 0.01 or 1. (C) Serial, 10-fold dilutions of the phages ФNM1 or ΦNM1Δgp11 spotted on lawns of cells harboring plasmid expressing Gp11 with 2.5 µM NaAsO2 or an empty vector (Vec). (D) Plaque morphologies of S. aureus RN4220 after infected with ФNM1, ФNM1Δgp11 (delete the entire gene gp11), or its complementary phage ФNM1Δgp11::gp11. For complementation assay, ФNM1Δgp11 was used to infect S. aureus RN4220, which contained the plasmid expressing gp11 with 2.5 µM NaAsO2. The experiments were repeated at least three times.