Fig 7.

Comparative analysis of novel gene essentialities in iRM23NL across four distinct growth media. (A) Classification of network-derived single gene deletions within iRM23NL, classified into essential, inessential, and partially essential genes, when subjected to aerobic (green) and anaerobic (orange) environments. Details regarding the classification schema can be found in Materials and Methods. (B) Protein sequence homology analysis of genes predicted to be essential in the laboratory media (LB and M9 pure supplemented with glucose) and the synthetically defined SNM and SCFM in both oxygen-rich and oxygen-limited conditions. The percentage identity threshold was set to 50% similarity to the human proteome.