Fig 1.

EBOV GP expression in cell lines with targeted disruptions in O-glycan biosynthesis. (A) Layout of Ebola virus glycoprotein. (B) Cartoon depiction of Ebola virus, as well as a ribbon diagram of a monomeric viral envelope glycoprotein (PDB: 6VKM). Color coding as in A. The mucin-like domain is not resolved and shown as a light-blue sphere. (C) Predominant O-glycosylation pathways in HEK293 cells. (D) Expression of GalNAc-T1, -T2, and -T3 in genetically engineered HEK293 cells. Scale bar, 5 µm. (E) Indicated cell lines transfected with a plasmid encoding full-length EBOV Makona GP were fixed with 4% PFA (Fix) 48 hours post-tranfection and co-stained for GP (red) and E-cadherin (green). Another set of cells was also permeabilized with 0.3% Triton X-100 (Fix & Perm). Scale bar, 10 µm.