TABLE 1.
O-glycosites and structures
| Glycosite | Recombinant GP PNA | Recombinant GP FT VVA | GalNAc-T regulation | Median KO/WT | VLPs PNA | VLPs FT VVA |
|---|---|---|---|---|---|---|
| Thr42 | Hex1HexNAc1 | HexNAc | ||||
| Thr77 | HexNAc | GalNAc-T1 | 0.43 | |||
| Thr83 | HexNAc | |||||
| Ser195 | Hex1HexNAc1 | |||||
| Thr206 | HexNAc | GalNAc-T1; GalNAc-T2 |
0.42; 0.27 | Hex1HexNAc1, Hex2HexNAc2 | HexNAc | |
| 283–284 | Hex1HexNAc1 | |||||
| Thr309 | Hex1HexNAc1, Hex1HexNAc2, Hex2HexNAc2 | Hex1HexNAc1, Hex2HexNAc2 | HexNAc | |||
| Ser312 | HexNAc, Hex1HexNAc1, Hex1HexNAc2, Hex2HexNAc2 | HexNAc | GalNAc-T1; GalNAc-T3 |
0.31; 0.33 | Hex1HexNAc1, Hex2HexNAc2 | HexNAc |
| Ser319 | HexNAc | |||||
| Ser322 | HexNAc, Hex1HexNAc1 |
|||||
| Thr326 | Hex1HexNAc1, Hex1HexNAc2, Hex2HexNAc2 |
HexNAc | HexNAc Hex1HexNAc1, Hex2HexNAc2 |
|||
| Ser327 | HexNAc, Hex1HexNAc1, Hex1HexNAc2, Hex2HexNAc2 |
GalNAc-T2 | 0.09 | HexNAc, Hex1HexNAc1, Hex1HexNAc2, Hex2HexNAc2 |
HexNAc | |
| Ser328 | HexNAc | HexNAc, Hex1HexNAc1, Hex2HexNAc2 |
||||
| Thr332 | HexNAc | HexNAc, Hex1HexNAc1, Hex2HexNAc2 |
||||
| Thr334 | Hex1HexNAc1, Hex1HexNAc2, Hex2HexNAc2 |
HexNAc | GalNAc-T1 | 0.19 | Hex2HexNAc2 | |
| Thr335 | Hex1HexNAc2 | HexNAc | ||||
| Ser344 | HexNAc, Hex2HexNAc2 | HexNAc, Hex1HexNAc1, Hex2HexNAc2 |
||||
| Ser347 | HexNAc, Hex1HexNAc1, Hex2HexNAc2 | HexNAc, Hex2HexNAc2 |
||||
| Ser348 | HexNAc, Hex1HexNAc1, Hex1HexNAc2, Hex2HexNAc2 |
HexNAc, Hex1HexNAc1, Hex2HexNAc2 |
GalNAc-T1 | 0.22 | HexNAc, Hex1HexNAc1, Hex2HexNAc2 |
HexNAc |
| Thr355 | Hex2HexNAc2 | HexNAc | HexNAc, Hex1HexNAc1, Hex2HexNAc2 |
|||
| Thr366 | HexNAc | HexNAc | HexNAc | |||
| Thr367 | HexNAc | HexNAc | Hex1HexNAc1 (1x) | HexNAc | ||
| Thr370 | HexNAc (2x) | HexNAc | HexNAc (1x) | |||
| Ser372 | Hex1HexNAc1 (1x) | |||||
| Thr373 | HexNAc | HexNAc | ||||
| Ser374 | Hex1HexNAc1 | HexNAc | HexNAc | |||
| Thr379 | HexNAc | HexNAc | Hex1HexNAc1 | HexNAc | ||
| Thr380 | HexNAc | HexNAc | HexNAc | |||
| Thr382 | HexNAc | HexNAc | ||||
| Thr388 | Hex1HexNAc1, Hex2HexNAc2 |
HexNAc | GalNAc-T1 | 0.47 | HexNAc | HexNAc |
| Thr391 | Hex1HexNAc1 | HexNAc | Hex1HexNAc1, Hex1HexNAc2, Hex2HexNAc2 |
HexNAc | ||
| Ser399 | HexNAc, Hex1HexNAc1, Hex1HexNAc2, Hex2HexNAc2 | HexNAc | HexNAc, Hex1HexNAc1, Hex1HexNAc2, Hex2HexNAc2 |
HexNAc | ||
| Thr402 | HexNAc, Hex1HexNAc1, Hex1HexNAc2, Hex2HexNAc2 | HexNAc | GalNAc-T1 | 0.43 | HexNAc, Hex1HexNAc1, Hex1HexNAc2, Hex2HexNAc2 |
HexNAc |
| Thr416 | HexNAc | HexNAc | HexNAc Hex1HexNAc1 |
HexNAc | ||
| Ser418 | HexNAc, Hex1HexNAc2 | HexNAc | GalNAc-T1 | 0.31 | HexNAc, Hex1HexNAc1 |
|
| Thr420 | Hex1HexNAc1 | HexNAc | ||||
| Thr424 | HexNAc, Hex1HexNAc1, Hex1HexNAc2, Hex2HexNAc2 | HexNAc | Hex1HexNAc1, Hex2HexNAc2 | HexNAc | ||
| Thr425 | HexNAc, Hex1HexNAc1, Hex1HexNAc2, Hex2HexNAc2 | HexNAc | HexNAc, Hex1HexNAc1, Hex2HexNAc2 |
HexNAc | ||
| Thr435 | HexNAc | |||||
| Ser438 | HexNAc, Hex1HexNAc1, Hex1HexNAc2, Hex2HexNAc2 | HexNAc | GalNAc-T1 | 0.13 | HexNAc, Hex1HexNAc1 |
HexNAc |
| Thr448 | Hex1HexNAc1 | HexNAc | ||||
| Thr449 | HexNAc | |||||
| Thr450 | HexNAc | |||||
| 455–456 (1x) | Hex1HexNAc1 | |||||
| Ser475 | HexNAc | GalNAc-T1 | 0.06 | |||
| Thr483 | HexNAc | Hex1HexNAc1, Hex2HexNAc2 |
||||
| Thr485 | Hex2HexNAc2 | HexNAc | Hex1HexNAc1, Hex2HexNAc2 | HexNAc | ||
| Thr494 | Hex1HexNAc1, Hex2HexNAc2 | HexNAc | GalNAc-T3 | 0.3 | Hex1HexNAc1, Hex2HexNAc2 | HexNAc |