Figure 4.

Reducing the level of IL-17a improves the anxiety state and social function of autism model mice. (A) Schematic diagram of the experiment. The animals were subjected to behavioral tests at 6 weeks. Anti-IL-17a antibody or sterile cerebrospinal fluid was injected into the prefrontal cortex, and the mice were subjected to behavioral tasks 3 days later. (B) WB detection of the relative expression levels and bands corresponding to IL-17a in the mPFC. Statistical results showed significant changes in IL-17a (n=3 in each group). (C) Protein blotting bands for IL-17a and TNF-α in the cerebellar region. (D) ELISA detection of IL-17a levels in serum. Statistical results showed significant changes in IL-17a (n=5 in each group). (E) Schematic diagram of the open field test in mice. Mice in each group were tested in the open field for 5 min. (F, G) Results of the open field test after intervention. Statistical results showed significant changes in the time spent in the center, and no significant difference in the distance (n=7 in each group). (H-K) Results of the three-chamber sociability test after intervention (n=7 in each group). (L) Heatmap of the three-chamber social experiment in mice.