Fig 3.

Phage and antibiotic combinations reduce the emergence of resistant strains of O157 more than either treatment alone. (A) Illustration of co-culture of phage and bacteria with or without adding fosfomycin. (B) Bacterial lysis curve under fosfomycin treatment. Fosfomycin with a final concentration of 4 µg/mL was added into the culture 1 h after bacterial addition. The same experiment was performed in five different runs. (C) Bacterial lysis curve under phage PP01 treatment. The same experiment was performed in three different runs. (D) Bacterial lysis curve under phage SP15 treatment. The same experiment was performed in three different runs. (E) Bacterial lysis under fosfomycin and phage PP01 treatment. The same experiment was performed in five different runs. (F) Bacterial lysis under fosfomycin and SP15 treatment. The same experiment was performed in five different runs. The phage at an MOI = 1 or fosfomycin at a final concentration of 4 µg/mL was added 1 h after bacterial addition.