Fig 1.

Expanding the glycoproteome of B. cenocepacia K56-2. (A) Glycopeptide analysis reveals the majority of B. cenocepacia K56-2 glycopeptides appear detectable across multiple culturing conditions. (B) Venn diagram of the overlap within the identified glycoproteins across different growth conditions supports FAIMS and HILIC enrichments and provide access to complementary regions of the glycoproteome. (C) Pie chart of identified glycoproteins reveals a total of 141 glycoproteins have now been identified across B. cenocepacia K56-2 and J2315. (D) EThcD MS2 of the novel glycoprotein BCAM0950 reveals two glycosylation sites within the glycopeptide ³⁹GTGAAGDAPEAAAVGGVAVAASQAAVPASAGVPPSLAGSSAPR⁸¹ with glycan-associated ions (204.09, 366.13, and 569.21 m/z, highlighted in blue) denoted according to the Symbol Nomenclature for Glycans.