Fig 5.

Complementation of ΔfliF and ΔmotB partially restores motility and protein levels. (A and B) Motility assays of complemented ΔfliF and ΔmotB demonstrate the restoration of motility by the expression of wild-type fliF or motB regardless of the absence or presence of the known glycosylation sites S₃₅₈ and S₃₃₁. (C and D) Proteomic analysis confirms the restoration of MotB and FliF protein levels within complemented strains. Induced samples are denoted by blue boxes, while non-induced samples are denoted by gray boxes. The plasmid contained within each strain is shown below the strain name. EV—empty pSCrhaB2, motB—pSCrhaB2-motB, motB_S331A—pSCrhaB2-motB_S331A, fliF—pSCrhaB2-fliF, and fliF_S358A—pSCrhaB2-fliF_S358A with the lower boundaries of the MaxLFQ shown defined by the imputed values observed within ΔfliF and ΔmotB EV, respectively.