Fig 6.
FliF and MotB contribute to motility in the absence of glycosylation. (A and B) Motility assays of complemented ΔpglLΔfliF and ΔpglLΔmotB compared to WT and ΔpglL demonstrate the restoration of motility by the expression of wild-type fliF or motB compared to EV. (C and D) Western analysis complemented strains demonstrate mobility shifts with FliF or MotB Alanine substitutions as well as FliF or MotB expressed within ΔpglL backgrounds. Induced samples are denoted by blue boxes, while non-induced samples are denoted by gray boxes. The plasmid contained within each strain is shown below the strain name. EV—empty pSCrhaB2, motB—pSCrhaB2-motB, motBS331A—pSCrhaB2-motBS331A, fliF—pSCrhaB2-fliF, and fliFS358A—pSCrhaB2-fliFS358A.