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. 2024 May 3;15(6):e00440-24. doi: 10.1128/mbio.00440-24

Fig 4.

Graphs plot soft agar diameter and biofilm mass for WT and mutant strains with various cheY and cheZ mutants. Western blot results from WT and mutant strains with anti-CheY are featured.

CheY phosphorylation is required for normal chemotaxis and biofilm formation. (A) Chemotaxis migration (colony diameter) in 0.35% soft agar plates after 3 days. Representative images of the halo formed by cognate strains in soft agar plates are shown at the top of panel A. (B) Western blot analysis using Anti-CheY antibody on 15% SDS-PAGE gels. Molecular weight markers are shown in the left lane of panel B. The arrow indicates the position of CheY, at the predicted molecular weight of 13.9 kD. (C) Biofilm formation of cheY and cheZ mutants on 96-well plates after culturing for 1 day. Biofilm mass was determined using crystal violet staining as in Fig. 1–3. In panels A and C, mutants showing significantly increased or decreased biofilm mass compared to WT were colored in red and blue, respectively. Data shown are the means ± SDs from at least three independent experiments. Statistical analyses were performed using ANOVA (****P < 0.0001), with asterisks indicating comparison to WT in each panel and ns indicating not significant.