Analysis of TRM cells in the peripheral blood and spleen after C. deneoformans infection OT-II mice were infected intratracheally with C. deneoformans. (A) Tm cells in CD4+ Tg-T and nonTg-T cells in the peripheral blood and spleen were analyzed using flow cytometry on days 0 (uninfected, n = 3) and 3 (n = 3) post-infection. (B) TRM cells in CD4+ nonTg-T cells in the peripheral blood, spleen, and lungs were analyzed using flow cytometry on days 0 (uninfected, n = 3) and 3 (n = 3) post-infection. Each column represents the mean ± SD. *, P < 0.05. Representative data demonstrating similar results from independent experiments are shown. Experiments were conducted once for day 0 and twice for day 3. The results for each tissue represent results from the same experiment.