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Pre-treatment groups of DNA extraction
| Group | Lysis | Pre-treatment |
|---|---|---|
| 1 | Chemical | Manufacturer’s protocol; incubation with proteinase K |
| 2 | Chemical | Manufacturer’s protocol; incubation without proteinase K |
| 3 | Chemical + mechanical | Bead plate + tissue lyser 15 Hz; 2 × 5 min |
| 4 | Chemical + mechanical | Bead plate + tissue lyser 15 Hz; 2 × 5 min + proteinase K incubation |
