TABLE 2.
Read counts of negative controls with V4 and V3V4a
| Negative controls, V4, read counts (paired, trimmed pairs) | |||||
|---|---|---|---|---|---|
| ID | Reads | ID | Reads | ID | Reads |
| DNA shield 1_1 | ✓ 2,863 | Omni 1_1 | ✓ 15,641 | EC 1_1 | ✓ 5,415 |
| DNA shield 1_2 | ✓ 3,271 | Omni 1_2 | !! 123,738 | EC 1_2 | ✓ 5,864 |
| DNA shield 2_1 | ! 26,052 | Omni 2_1 | ✓ 15,595 | EC 2_1 | ! 34,811 |
| DNA shield 2_2 | ! 34,934 | Omni 2_2 | ! 25,796 | EC 2_2 | ! 33,879 |
| DNA shield 3_1 | ! 40,892 | Omni 3_1 | ! 39,777 | EC 3_1 | ! 57,900 |
| DNA shield 3_2 | ! 54,439 | Omni 3_2 | ! 36,932 | EC 3_2 | ✓ 15,211 |
| DNA shield 4_1 | ✓ 21,053 | Omni 4_1 | ✓ 19,867 | EC 4_1 | ✓ 2,427 |
| DNA shield 4_2 | ! 31,600 | Omni 4_2 | ✓ 6,395 | EC 4_2 | ✓ 16,672 |
| PCR-0_V4 | ✓ 13,657 | ||||
a
DNA shield, DNA/RNA shield fluid; Omni, OMNIgeneGUT; EC, extraction control/lysis buffer; first number, pre-treatment group; second number, number of replicate, e.g., EC1_2 is an extraction control with pre-treatment 1 and second replicate. The symbols indicate the number of reads: ✓ < 25,000; 25,000 < ! < 100,000; !! > 100,000 reads.