TABLE 3.

Primers and probes for the identification of the common and occasional causative agents of eumycetoma

Species	Primer/probe name	Sequence	Reference
Madurella mycetomatis	26.1a	5′-AATGAGTTGGGCTTTAACGG-3′	(224)
	28.3a	5′-TCCCGGTAGTGTAGTGTCCCT-3′	(224)
	mm-fw	5′-TCTCCTGTCCTACGACATCTGTGG-3′	(225)
	mm-rv	5′-TTCCTCACCTCCCAGCCCTTT-3′	(225)
	PF2-specific-Myc	5’-TGACCGTCGGCGTCTCTT-3’	(226)
	R2-specific-Myc	5′-TAGGCTGTCAGAAAACACATCG-3′	(226)
	PF3-Myc	5'-CTCCCGGTAGTGTAGTGT-3’	(226)
	R3-Myc	5'-CAGAAGACTCAGAGAGGCC-3’	(226)
	M.m_forward	5′-CCTCCCGGTAGTGTAGTGTCC-3′	(227)
	M.m_reverse	5′-GAGAGGCCGTACAGAGCAAAT-3′	(227)
	M.m_probe	5′-FAM-GGCGTCCGCCGGAGGATTATACAAC-BHQ1-3′	(227)
Falciformispora senegalensis	F.s_forward	5′-GTTCCTACGCCGGCAAC-3′	(227)
	F.s_reverse	5′-AGACAGGTATACTGCTTTTGCTGC-3′	(227)
	F.s_probe	5′-HEX- GCCGCTGGGTCTCCACC-BHQ1-3′	(227)
Scedosporium boydii	Forward	5′-TGGCGAGCACGGTCTTG-3′	(228)
	Reverse	5′-ACATTCACGGCAGACACTGATT-3′	(228)
	Apioboy_P	5'-FAM-TAGCAACGGAGTGTACGGAACCACCC-BBQ-3’	(228)