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. 2024 May 2;15(6):e00339-24. doi: 10.1128/mbio.00339-24

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Copyright © 2024 Watanabe et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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Graphs plot intracellular levels of ribonucleoside G, A, C, and U and deoxyribonucleoside dG, dA, dC, and dT for each phosphate group in six JMUB217 mutants with reduced oxacillin susceptibility. — Ribo/deoxyribonucleotide profile of JMUB217-derived mutants. Quantitative values of each ribo/deoxyribonucleotide of JMUB217-derived mutants with reduced oxacillin susceptibility: rpiA (JMUB217-7 RpiA^A64E), fruB (JMUB217-13 FruB^A211E), rpoB1 (JMUB217-23 RpoB^H929P), rpoB2 (JMUB217-22 RpoB^Q645H), rpoC1 (JMUB217-19 RpoC^G950R), and rpoC2 (JMUB217-24 RpoC^G498D). The y-axis represents the amount of intracellular ribo/deoxyribonucleotide (pmol) in 1 mL of cell suspension at OD₆₀₀ = 1. Data represent means with standard error from three independent experiments. N.D., not detected. Mean values of intracellular ribo/deoxyribonucleotide of mutant strains were compared with that of wild-type via one-way ANOVA. *P < 0.05, **P < 0.01, and ***P < 0.001. Figures 3 to 5 and Fig. S1 were constructed from data of the same metabolomics analysis.