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. 2024 May 2;15(6):e00339-24. doi: 10.1128/mbio.00339-24

Fig 7.

The results for rpoB2 and rpoC2 mutants with/without aTc induction of cUMP and cCMP synthase genes are featured. Graphs plot UTP and UMP of rpoB2 mutants in the presence/absence of aTc induction.

Altered oxacillin susceptibility by cUMP/cCMP gene expressions in JMUB217-derived rpoBC mutants. (A) Oxacillin susceptibility of cUMP/cCMP gene-expressing rpoB2 and rpoC2 mutants tested by E-test. Oxacillin susceptibility of S. aureus JMUB217-22 (rpoB2) and JMUB217-24 (rpoC2) carrying an aTc-inducible pLC1t2 plasmid containing JMUB217-derived cUMP and JMUB4998-derived cCMP synthase genes, respectively, was measured by oxacillin E-test. The concentration of aTc used for the induction was 50 ng/mL. (B) Relative abundances of intracellular UTP and UMP of S. aureus JMUB217-22 (rpoB2) harboring pLC1t2_cUMP synthase in the presence/absence of inducer, 50 ng/mL aTc. Data represent means with standard error from three independent experiments.