Fig. 3.

DHT-mediated changes in BAT adipogenesis and de novo lipogenesis are modulated by miR-21. WT or miR21KO mice were treated with dihydrotestosterone (DHT) or vehicle (Veh) for 90 days. C/EBPα (Cebpa, A), C/EBPβ (Cebpb, C), Adiponectin (Adipoq, E), and PPARγ (pparg, G) mRNA was quantified by RT-qPCR and standardized to the geometric mean of four reference genes (HGMK) (N = 6/group). C/EBPα (B), C/EBPβ (D), Adiponectin (F), PPARγ (H), ACC (I), FAS (J), Fabp4 (K), and Perilipin 1 (L) protein was quantified by Western-blot and normalized to GAPDH (N = 4 mice/group). Data are expressed as mean ± SEM. Data were analyzed by two-way ANOVA followed by Fisher’s LSD test. *p < 0.05; **p < 0.01; ***p < 0.001. ACC acetyl-CoA carboxylase, FAS fatty acid synthase, Fabp4 fatty acid binding protein 4