Fig. 1.

General overview of the three-step strategy. In the first step (A), transcriptomics data are integrated to a GSMN with a partial enumeration approach adapted from DEXOM. In the second step (B), DARs are computed from the large number of metabolic networks obtained after the optimization and sampling step. Finally, in the third step (C), network analysis methods are developed and employed to interpret DARs and improve our understanding of the chemicals’ mMoA