FIG. 1.

Inhibition of infection of SK6 cells with CSFV by GAGs. The numbers of plaques in 2-cm² tissue culture wells were measured in a plaque assay; SK6 cells were preincubated for 30 min with 100 μl of medium with different concentrations of GAGs. Subsequently, 100 μl of a virus dilution containing about 1,000 PFU of CSFV strain Brescia clone C1.1.1 was added to the wells. The cells were incubated for 30 min, washed, and supplied with overlay medium. After incubation for 24 h the cells were immunostained and the plaques were counted. The x axis represents the concentration of GAGs present during the 30 min of virus adsorption (a twofold-lower concentration of GAGs than in the preincubation solution). Plot symbols represent the mean of two independent observations, and the error bars represent the variation between these two observations.