FIG. 4.

T3 reovirus induces G₂/M arrest in murine, canine, and human cells. Asynchronous, subconfluent monolayers of L929 (A), MDCK (B), C127 (C), HEK293 (D), and HeLa (E) cells were either mock infected (white) or infected with T1L (gray) or T3A (black) at an MOI of 100 PFU per cell. Cells were harvested at 48 h postinfection, stained with Krishan's stain, and analyzed for DNA content using flow cytometry. Results are presented as the mean percentage of cells in G₂/M phase for three independent experiments. The error bars indicate the standard errors of the mean. A significantly greater percentage of T3A-infected cells were in G₂/M than mock-infected cells in all cell lines tested (P < 0.01 to 0.001). A significantly greater percentage of T3A-infected cells were in G₂/M than T1L-infected cells in all cell lines tested (P < 0.01 to 0.001) except HeLa. A significantly greater percentage of T1L-infected cells were in G₂/M than mock-infected cells in L929 and HEK293 cells (P < 0.001).