FIG. 7.

ς1s expression induces an increase in the percentage of cells in G₂/M phase. C127 cells stably transfected with ς1s (BPX-6) or vector control (BPV-12) under the control of the mouse metallothionein promoter were induced with CdCl₂, harvested at the indicated times postinduction, and analyzed for DNA content by flow cytometry. The results are presented as the mean percentage of cells in the G₂/M phase of the cell cycle for three to six independent experiments. The error bars indicate the standard errors of the mean. The percentage of cells in G₂/M was significantly greater in the ς1s-expressing cells than in the vector-control cells at 45 h (P = 0.03, n = 4) and 55 h (P = 0.005, n = 6) postinduction.