FIG. 8.

Inhibitors of reovirus-induced apoptosis do not inhibit reovirus-induced G₂/M arrest. (A) Effect of calpain inhibitor PD150606 on T3A-induced G₂/M arrest. L929 cells were treated with either 25 μM calpain inhibitor PD150606 or an ethanol control and then either mock infected or infected with T3A at an MOI of 100 PFU per cell. (B) Effect of caspase 3 inhibitor DEVD-CHO on T3A-induced G₂/M arrest. HEK293 cells were treated with either 100 μM caspase 3 inhibitor DEVD-CHO or a dimethyl sulfoxide control and then either mock infected or infected with T3A at an MOI of 100 PFU per cell. (C) Effect of anti-TRAIL antibodies on T3A-induced G₂/M arrest. HEK293 cells were treated with either 30 μg of an anti-TRAIL antibody per ml or mock treated as a control and then either mock infected or infected with T3A at an MOI of 100 PFU per cell. (D) Effect of NF-κB inhibition on T3A-induced G₂/M arrest. HEK293 cells expressing a dominant-negative form of IκB (IκB-ΔN2) to inhibit NF-κB activation or untransfected HEK293 cells were either mock infected or infected with T3A at an MOI of 100 PFU per cell. In all cases, G₂/M arrest was assessed 48 h postinfection.