. Author manuscript; available in PMC: 2024 Jul 11.

Published in final edited form as: Biodes Manuf. 2023 Oct 3;6(6):646–660. doi: 10.1007/s42242-023-00255-1

Table 1.

Typical cell sources and ECMs used for establishing human lung organoid cultures.

Human lung organoid	Cell source	ECM	Reference
Airway and bronchial organoid	Solid human lung tissue	Matrigel	[Zhou 2018, Sachs 2019]
	Human lung fibroblasts (HLF)	Matrigel	[Tan 2017]
	Human lung microvascular lung endothelial cells (HMVEC-L)
	Human bronchial epithelial cells (NHBEs)
	NHBEs	Matrigel	[Sano 2022]
	Human bronchial epithelial cells (hBEpCs)	N.A.	[Fang 2021]
Alveolar organoid	Human pluripotent stem cells (hPSCs)	Matrigel	[Han 2021, Han 2022]
	Induced pluripotent stem cells (iPSCs)	Matrigel	[Tiwari 2021]
	Immortalized human alveolar epithelial cells	Matrigel	[Tran 2022b]
Bronchioalveolar organoid	Human small airway stem cells	Matrigel, collagen I	[Lamers 2021]