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. Author manuscript; available in PMC: 2024 Aug 9.
Published in final edited form as: Biomater Sci. 2024 Jul 9;12(14):3633–3648. doi: 10.1039/d4bm00391h

Fig. 3.

Fig. 3

Cellular uptake and compatibility of ICG/BPs. After 24 hours of co-culturing BMSCs with ICG/BPs, the cellular uptake of the particles was analysed using (A) confocal laser scanning microscopy and (B) TEM imaging. (C) The percentage of cellular uptake was determined by flow cytometry after co-culturing BMSCs with ICG/BPs for 24 and 48 hours. (D) After 4 weeks of cell culture, the cytotoxicity of the ICG/BPs was determined by Live/Dead staining and osteogenic differentiation was evaluated with RUNX2 and OCN staining, and calcium deposition measured with alizarin red S staining.