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. Author manuscript; available in PMC: 2024 Jul 11.
Published in final edited form as: ACS Nano. 2022 Mar 24;16(4):5335–5348. doi: 10.1021/acsnano.1c04303

Figure 2.

Figure 2.

Cardiomyocytes display elongated morphology with integrin–ligand tension > 12pN. (a) Representative images of CMCs (live) on 12, 56, and 160 pN rupture probe surfaces; the leftmost panel (RICM) channel shows the outline of the attached cells, and the middle panel (bright field), TRITC channel, shows the signal increase of Cy3B fluorescence due to probe rupture for 12 and 56 pN probes and the inverted fluorescence loss for 160 pN probes (t = 6–8 h. (b–f) Bar graphs showing the spread area, aspect ratio (x/y), circularity, and mean fluorescence under the cells and integrated fluorescent density obtained from CMCs that were cultured on 12, 56, and 160 pN probes. Each data point represents a single cell while the bar shows the average. ****, ***, **, *, and ns indicate p < 0.0001, p < 0.001, p < 0.01, p < 0.05, and not significant, respectively, as determined from one-way ANOVA. Error bars show standard deviation for n > 3, where each experiment was averaged from three or more different cell isolations with three different sets of surface preparations. Scale bar = 20 μm.