Skip to main content
. Author manuscript; available in PMC: 2024 Jul 11.
Published in final edited form as: Nature. 2023 Aug 30;621(7977):179–187. doi: 10.1038/s41586-023-06483-w

Figure 4. Pdss2 expression is required and sufficient to promote mitochondrial respiration and CoQ synthesis in CD8 T cells.

Figure 4.

a, Oxygen consumption rate (OCR) of in vitro activated shCd19 and shSrebf2 CD8 T cells subjected to the MitoStress test (Seahorse). Oligomycin (O), FCCP (F), Rotenone/Antimycin A (R/A). b, Oxygen consumption rate (OCR) of in vitro activated CD8 T cells treated with statin (simvastatin) in combination with mevalonolactone (MVA) or cholesterol (Chol.) and subjected to the MitoStress test (Seahorse). c, OCR of in vitro-activated sgCd19 and sgPdss2 Cas9eGFP CD8 T cells subjected to the MitoStress test (Seahorse). d, OCR of in vitro activated sgCd19 and sgFdft1 Cas9eGFP CD8 T cells subjected to the MitoStress test (Seahorse). e, OCR of in vitro activated sgCd19, sgFdft1, and sgFdft1/Pdss2 Cas9eGFP CD8 T cells subjected to the MitoStress test (Seahorse). f, OCR of in vitro activated empty vector control (Empty) and Pdss2 OE CD8 T cells subjected to the MitoStress test (Seahorse). g, Quantification of CoQ and demethoxyubiquinone (DMQ) species in in vitro activated sgCd19 and sgFdft1 Cas9eGFP CD8 T cells normalized to total protein content. h, Quantification of CoQ and DMQ species in in vitro activated Empty and Pdss2 OE CD8 T cells normalized to total protein content. Data are mean +/− s.e.m. and representative of at least two independent experiments (a-h), with a total of n=4 (a), n=5 (b), n=3 (c), n=2 (e), n=3 (f), n=3 (g), n=5 (h) cell replicates. Two-way ANOVA (a, c, d, e, and f). Two-sided unpaired t-Test (b, g, and h) *P<0.05, **P<0.01, ***P< 0.005.