FIG. 2.

SDS-polyacrylamide gel electrophoresis separation of ³⁵S-labeled proteins synthesized in VSV-infected MEFs from PKR^−/− and control animals. MEFs were either not infected (−) or infected with VSV at an MOI of 3 for 2, 4, 6, or 8 h as described in Materials and Methods. The infected cells at each time point were pulsed with [³⁵S]methionine-cysteine for 1 h prior to being harvested and lysed. VSV proteins (G, N, and M) begin to appear in PKR^−/− and control cells at 4 h p.i. While this pattern of labeled proteins remains constant in control cells up to 8 h p.i. viral proteins become the predominant translated proteins in the PKR^−/− samples by 8 h p.i. Molecular masses are indicated at left of each panel in kilodaltons.