Table 1. Factor and levels of formulation.

		Factor 1	Factor 2	Response 1	Response 2
Std	Run	A:Ca3(PO4) (gm)	B:Trehalose (mg)	Entrapment efficacy %	In vitro drug release %
6	1	4.4	150	19.2	47.5
2	2	4	100	19	45.3
12	3	3	150	23	57.2
7	4	3	79	21.2	48.2
4	5	4	200	19.2	50.2
8	6	3	220	23.4	62.3
11	7	3	150	23.4	57.3
3	8	2	200	35	62
10	9	3	150	23.1	57.2
1	10	2	100	30	50
5	11	1.5	150	21	55
13	12	3	150	23	57
9	13	3	150	23.2	57.3

Factor 1: Represents the first factor studied in the experiment (A:Ca3(PO4)). Factor 2: Represents the second factor studied in the experiment (B: Trehalose). Std Run: Standard Run number for the experiment. A:Ca3(PO4): Amount of Ca3(PO4) used in the formulation. B: Trehalose: Amount of Trehalose used in the formulation. Response 1: Entrapment efficacy of the aquasome formulation. Response 2: In vitro drug release from the aquasome formulation.

%: Percentage; gm: Gram; mg: Milligram.