Table 2. Response surface regression: analysis of variance.
| Response 1: entrapment efficacy | ||||||
|---|---|---|---|---|---|---|
| Source | Sum of squares | df | Mean square | F-value | p-value | |
| Model | 116.28 | 2 | 58.14 | 4.56 | 0.0391 | Significant |
| A-Ca3(PO4) | 107.65 | 1 | 107.65 | 8.44 | 0.0157 | |
| B-Trehalose | 8.63 | 1 | 8.63 | 0.6772 | 0.4297 | |
| Residual | 127.51 | 10 | 12.75 | |||
| Lack of fit | 127.40 | 6 | 21.23 | 758.33 | <0.0001 | significant |
| Pure error | 0.1120 | 4 | 0.0280 | |||
| Cor total | 243.79 | 12 | ||||
| Response 2: in vitro drug release | ||||||
|---|---|---|---|---|---|---|
| Source | Sum of squares | df | Mean square | F-value | p-value | |
| Model | 356.27 | 5 | 71.25 | 53.41 | <0.0001 | Significant |
| A-Ca3(PO4) | 91.85 | 1 | 91.85 | 68.85 | <0.0001 | |
| B-Trehalose | 169.65 | 1 | 169.65 | 127.17 | <0.0001 | |
| AB | 12.60 | 1 | 12.60 | 9.45 | 0.0180 | |
| A2 | 76.62 | 1 | 76.62 | 57.43 | 0.0001 | |
| B2 | 12.10 | 1 | 12.10 | 9.07 | 0.0196 | |
| Residual | 9.34 | 7 | 1.33 | |||
| Lack of fit | 9.28 | 3 | 3.09 | 206.18 | <0.0001 | Significant |
| Pure error | 0.0600 | 4 | 0.0150 | |||
| Cor total | 365.61 | 12 | ||||
Statistical analysis results showing the significance of factors A (Ca3(PO4)) and B (Trehalose) on Response 1 (Entrapment efficacy) and Response 2 (in vitro drug release), with p-values indicating significance levels.
Ca3(po4): Calcium phosphate; df: Degree of freedom.