FIG. 1.

Purification of MBP-nsp10 and MBP-nsp10-KQ from E. coli lysates. The MBP fusion proteins were purified by affinity chromatography as described in Materials and Methods. An SDS–10% polyacrylamide gel stained with Coomassie brilliant blue dye is shown, and the position of the recombinant 93-kDa proteins is indicated by an arrowhead. Lanes: M, protein molecular mass markers (with masses, in kilodaltons, indicated on the left); 1, total lysate from E. coli cells transformed with pMal-nsp10; 2, total lysate from IPTG-induced E. coli cells transformed with pMal-nsp10; 3, 3 μg of amylose affinity-purified MBP-nsp10 protein; 4, total lysate from E. coli cells transformed with pMal-nsp10-KQ; 5, total lysate from IPTG-induced E. coli cells transformed with pMal-nsp10-KQ; 6, 3 μg of amylose affinity-purified MBP-nsp10-KQ protein.