FIG. 2.

ATPase activity of MBP-nsp10. The ATPase activity was analyzed by thin-layer chromatography using [γ-³²P]ATP as a substrate as described in Materials and Methods. The positions of ATP and inorganic phosphate (P_i) are indicated. Lanes: 1, reaction without protein; 2, reaction containing 600 fmol of MBP-nsp10; 3, reaction containing 600 fmol of MBP-nsp10-KQ; 4, reaction containing 600 fmol of MBP-nsp10 and 1 μg of poly(U) per ml; 5, reaction containing 600 fmol of MBP-nsp10 and 150 μg of poly(U) per ml; 6, reaction containing 600 fmol of MBP-nsp10-KQ and 150 μg of poly(U) per ml.