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. Author manuscript; available in PMC: 2024 Jul 11.
Published in final edited form as: Methods Enzymol. 2022 Nov 26;678:331–350. doi: 10.1016/bs.mie.2022.09.022

Fig. 4.

Fig. 4

AIF HT-SAXS Screening Analysis of 14 Focused Hits. (A) Workflow for triaging and analyzing large HT-SAXS screening datasets. (B) I(0), Rg, and VR results from the focused 14-fragment AIF SAXS screen. Gray and red lines indicate average and three-standard deviations from AIF-DMSO controls. (C) Overlay of normalized Kratky transforms from A8 (blue) and AIF monomer (gray) and dimer (black) benchmarks. (D) SDS-PAGE of BS3 amine-crosslinking reactions that capture AIF dimers in the presence of NADH among top candidates from MST screening. D, dimer; M, monomer.