Fig. 6. Single-particle reconstruction of the Aca2–RNA complex with a molecular weight of 40 kDa.

a, Local resolution of the reconstruction with Blush regularization. b, Automated atomic model assignment with ModelAngelo. c, Detailed view of an α-helical segment in the reconstructed map and refined atomic model. d, The solid line shows the FSC to a reference atomic model, and the dashed line shows the half-map FSC. The solvent-corrected resolution is 2.5 Å, using a spectral trailing cut-off at 3.0 Å. e, Processing pipeline from initial model to final reconstruction. Numbers indicate the number of particles assigned to each map. Purple squares indicate reconstructions using Blush regularization.