Extended Data Fig. 3. Averaged amplitudes and stability of facilitating, depressing and stable connections, and input summation at LA-LA synapses.

a, Connections could be characterized as facilitating (red), depressing (blue) or stable (grey). Top, examples for each connection type, with each example displaying the respective recordings of presynaptic action potentials and postsynaptic uEPSPs (average of 15 sweeps). Bottom, Averaged uEPSP amplitudes for 1st through 8th and ‘recovery’ stimuli; for n = 29 facilitating, 12 depressing and 41 stable connections providing additional information to Fig. 3d. Friedman test on repeated measures with Bonferroni post-hoc comparisons. * P = 0.019, *** P < 0.0001. Bars are means + SEM. b, Example of input summation at a triple convergent motif. Three presynaptic neurons (blue traces and blue cells; numbered i, ii and iii) converge onto one postsynaptic neuron (black traces and black cell; numbered iv). Individual presynaptic stimulation of each blue cell (i-iii) at 20 Hz leads to a postsynaptic facilitating response (iv). The arithmetic summation (+signs) of all black responses leads to (=sign) the summed EPSP response that results from stimulating all connections simultaneously – see⁴³. This convergent motif is an example of the summed EPSPs that are used in Fig. 3e (right panel; black and red dots).