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. 2024 Jun 13;27(7):1309–1317. doi: 10.1038/s41593-024-01676-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — a, Microdrive with 8 tetrodes (32 electrodes total) used for in vivo electrophysiological recordings in freely-moving rats. b, Optical fiber that allows – in addition to recording – stimulation with blue light. c, Rat implanted with a microdrive. d, DAPI staining of a coronal section of the lateral amygdala (inset: LA, dashed outline) showing the localization of the implanted tetrodes (black arrows). Image shows a representative example of tetrode or optrode placements in 11 rats. Scale bars indicate 2 mm or 0.5 mm (inset). e, Localization of all electrode tips, determined post-hoc. Scale bar indicates 1 mm. f, Example of Channelrhodopsin-2-expressing neuronal unit responding to a 1-ms blue-light pulse with time-locked spikes following the blue stimulus within a few ms (top, rasterplot data, n = 540 repetitions; bottom, z-score representation with the horizontal dashed line representing 3 standard deviations of baseline activity); inset: spike waveforms of recorded unit.