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. 2024 Jul 2;27(7):1260–1273. doi: 10.1038/s41593-024-01677-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — (a) Bar plot showing the number of cells per experimental condition that passed the quality control filters and were used for downstream analysis. (b) Violin and box-whisker plot depicting the number of UMIs per cell for each experimental condition (n = 3666, 3608, 3991 and 3693 cells per condition respectively). (c) Same as d, but for each annotated cell-type cluster (n = 6790, 338, 2378, 3691, 835, 175, 444, 208 and 99 cells per cluster respectively). (d) Violin and box-whisker plot depicting the number of genes per cell for each experimental condition (n = 3666, 3608, 3991 and 3693 cells respectively). (e) Same as f, but for each annotated cell-type cluster (n = 6790, 338, 2378, 3691, 835, 175, 444, 208 and 99 cells per cluster respectively). (f) Volcano plot showing the differentially expressed genes for the following pairwise comparison, Yy1_KO/Ngn2+ vs Yy1_WT/Ngn2. Yellow, red, and grey dots represent the up-, down- and non-regulated genes. (g) UMAP visualization of the expression levels of the indicated marker genes. All boxplots display median (line), 25th or 75th percentiles (box),10th or 90th percentiles (whisker) and outliers (dots).